Differentiating Schistosoma haematobium from Related Animal Schistosomes by PCR Amplifying Inter-Repeat Sequences Flanking Newly Selected Repeated Sequences
نویسندگان
چکیده
منابع مشابه
Directional PCR cloning of multiple repeat sequences.
The tandem ligation of DNA sequences within plasmid vectors is hindered by the inability to force the orientation of subsequently ligated DNA fragments and the inherent instability of repeated sequences when grown in bacterial hosts. Improvements have been made in the latter by the introduction of several E. coli strains by commercial suppliers (STBL2; Life Technologies, Gaithersburg, MD, USA o...
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a Department of Zoology, James Cook University, Townsville 4811, Queensland, Australia b Department of Population Genetics, Primate Research Institute, Kyoto UniversiO', Kanrin, lnuyama 484, Japan ~ Department of Anatomy, Kochi Medical School, Nankoku City, Kochi 783, Japan d Department of Parasitology, Facuhy of Medicine, Fukuoka UniversiO,, Jyonan-ku, Fukuoka City, Fukuoka 814-01, Japan Depar...
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The availability of the Tropheryma whipplei genome offers the putative possibility of choosing logical DNA targets. We applied a PCR assay (targeting repeated sequences of T. whipplei) to samples from patients with Whipple's disease and to those from members of a control group. When compared to the results seen with regular PCR, the sensitivity of repeat PCR was significantly enhanced (P = 0.02...
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The present study describes a real-time PCR approach with high resolution melting-curve (HRM) assay developed for the detection and differentiation of Schistosoma mansoni and S. haematobium in fecal and urine samples collected from rural Yemen. The samples were screened by microscopy and PCR for the Schistosoma species infection. A pair of degenerate primers were designed targeting partial regi...
متن کاملHybrid DNA artifact from PCR of closely related target sequences.
We have recently determined through standard molecular cloning methods that Xenopus laevis contains two different nonallelic preproinsulin genes which are very similar to each other (i.e. 94% identity in the coding region) (1). In order to obtain the remaining sequence of the 5'-untranslated regions we used the anchored polymerase chain reaction method as described by others (2,3). We first rev...
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ژورنال
عنوان ژورنال: The American Journal of Tropical Medicine and Hygiene
سال: 2012
ISSN: 0002-9637,1476-1645
DOI: 10.4269/ajtmh.2012.12-0243